DNA Sequence Reverse Complement Calculator

A reverse complement is created by first reversing the DNA sequence (reading it backwards) and then replacing each nucleotide with its complement (A↔T, G↔C). This is important for analyzing both strands of double-stranded DNA.

Reverse complements are essential when analyzing open reading frames (ORFs) on the reverse strand, designing PCR primers, analyzing palindromic sequences, or working with antisense DNA strands.

Reverse reads the sequence backwards, complement changes each base to its pair (A↔T, G↔C), and reverse complement does both operations - first reverse then complement the sequence.

The calculator accepts standard DNA nucleotide codes: A (Adenine), T (Thymine), G (Guanine), and C (Cytosine). Other characters will be ignored or may cause errors.

To find ORFs on the reverse strand, use the reverse complement option to get the sequence as it would appear on the opposite strand, then search for start codons (ATG) and stop codons in the resulting sequence.

GC content is the percentage of guanine (G) and cytosine (C) bases in the DNA sequence. It affects DNA melting temperature, primer design, and can indicate functional regions in genomic sequences.

This tool is designed for DNA sequences. For RNA sequences, you would need to replace uracil (U) with thymine (T) first, or use a specialized RNA tool that handles U↔A base pairing.

While there's no strict limit, very long sequences may take longer to process. The tool works best with sequences up to several thousand base pairs for optimal performance.