Enter your DNA or RNA sequence into the GC Content Calculator and select your sequence type to find the GC content percentage, plus a full nucleotide breakdown showing your sequence length and individual counts for G (Guanine), C (Cytosine), and A (Adenine).
GC Content
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Sequence Length
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G (Guanine) Count
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C (Cytosine) Count
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A (Adenine) Count
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T/U Count
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Other Bases
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GC content refers to the percentage of guanine (G) and cytosine (C) bases in a DNA or RNA sequence. It's an important measure in molecular biology as it affects the stability and melting temperature of the nucleic acid.
Simply paste your DNA or RNA sequence into the text area, select the sequence type (DNA or RNA), and the calculator will automatically compute the GC percentage and base composition statistics.
GC content affects the melting temperature (Tm) of DNA primers and templates. Higher GC content leads to higher melting temperatures, which is crucial for designing PCR primers and optimizing reaction conditions.
The calculator accepts both FASTA format and plain text sequences. It recognizes standard nucleotide characters: A, T, G, C for DNA and A, U, G, C for RNA.
CpG islands are regions of DNA with high GC content, typically above 50%. They are important in gene regulation and are often found in promoter regions of genes.
Yes, the calculator can analyze both DNA and RNA sequences. For RNA, it will count uracil (U) instead of thymine (T) and adjust the analysis accordingly.
The calculator will count any non-standard nucleotide characters as 'Other' bases and exclude them from the GC content calculation, focusing only on valid A, T/U, G, and C bases.