Enter any three known values — Initial Concentration (C1), Final Concentration (C2), and either Volume to Use (V1) or Final Volume (V2) — and the Cell Dilution Calculator solves the missing variable using C1V1 = C2V2, giving you the Volume of Stock Solution Needed, Dilution Factor, and Volume of Diluent to Add.
Volume of Stock Solution Needed
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Dilution Factor
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Volume of Diluent to Add
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C1V1=C2V2 is the dilution equation where C1 is initial concentration, V1 is volume of stock solution needed, C2 is final concentration, and V2 is final volume. This formula is based on the principle that the amount of solute remains constant during dilution.
Enter your stock concentration (C1), desired final concentration (C2), and final volume needed (V2). The calculator will determine how much stock solution (V1) you need to achieve the desired dilution.
The dilution factor is the ratio of initial concentration to final concentration (C1/C2). For example, diluting from 10 mg/ml to 1 mg/ml gives a dilution factor of 10×, meaning the solution is diluted 10-fold.
Yes, this calculator supports various units including mg/ml, µg/ml, molar concentrations (mM, µM, nM), and cell concentrations. Just ensure both C1 and C2 use the same units for accurate calculations.
The diluent volume equals the final volume minus the stock volume (V2 - V1). This calculator automatically shows you how much diluent to add to achieve your target dilution.
If you know V1 (stock volume), leave that field filled and the calculator will determine what final concentration you'll achieve with your desired final volume.
Accurate dilution ensures proper cell density for experiments, maintains cell viability, and provides reproducible results. Incorrect dilutions can lead to experimental failure or unreliable data.