Centrifugation RCF/RPM Calculator

In centrifugation, RCF (Relative Centrifugal Force) measured in × g tells you the actual force applied to your sample, while RPM (revolutions per minute) describes how fast the rotor spins — and converting between them requires knowing the rotor size. Select whether you want to calculate RCF from RPM or RPM from RCF, then enter your known value and rotor radius to get the converted result along with the formula used.

What do you want to calculate? *

rpm
x g
cm

Distance from center of rotation to sample

Results

Calculated Value

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Formula Used

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Frequently Asked Questions

What is Centrifugation?

Centrifugation is a process that uses centrifugal force to separate components of different densities within a liquid. It's commonly used in laboratories to separate cells, proteins, DNA, and other biological materials.

What is Relative Centrifugal Force (RCF)?

RCF is the centrifugal force expressed as multiples of Earth's gravitational force (g). It provides a standardized way to describe centrifuge conditions regardless of the specific centrifuge model or rotor used.

What do RCF and RPM mean?

RCF (Relative Centrifugal Force) is the force applied to samples as a multiple of gravity, while RPM (Revolutions Per Minute) is the rotational speed. RCF is the preferred unit because it accounts for rotor radius and provides consistent results across different centrifuges.

What is the difference between RPM and RCF, and why does it matter?

RPM measures rotational speed, while RCF measures the actual force applied to samples. The same RPM can produce different RCF values depending on rotor radius. Using RCF ensures reproducible results across different centrifuges and protocols.

How do I find my centrifuge rotor radius?

The rotor radius is typically listed in your centrifuge manual or on the rotor itself. It's measured from the center of rotation to the center of the sample tube. Different rotors have different radii, so always check your specific rotor specifications.

Why is rotor radius important in centrifugation calculations?

Rotor radius directly affects the centrifugal force applied to samples. A larger radius generates more force at the same RPM. This is why protocols should specify RCF rather than RPM for reproducible results.

What is the formula for converting RPM to RCF?

The formula is: RCF = (RPM)² × 1.118 × 10⁻⁵ × r, where r is the rotor radius in centimeters. This formula accounts for both rotational speed and the distance from the center of rotation.

Can I use this calculator for any type of centrifuge?

Yes, this calculator works for any centrifuge as long as you know the rotor radius. The physics of centrifugal force is the same whether you're using a microcentrifuge, benchtop centrifuge, or high-speed ultracentrifuge.