Dilution Factor Calculator

In lab work, a dilution factor tells you how much a concentrated solution has been weakened — and getting it right matters for experiments, assays, and reagent preparation. Enter your Volume from Stock Solution (V₁) and Final Solution Volume (V₂) into the Dilution Factor Calculator to get the Dilution Factor, along with the Dilution Ratio, Fold Dilution, and the exact Diluent Volume Required to reach your target volume.

mL

Volume to be removed from the concentrated stock solution

mL

Total volume after dilution (stock + diluent)

Results

Dilution Factor

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Dilution Ratio

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Fold Dilution

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Diluent Volume Required

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Frequently Asked Questions

What is dilution factor?

Dilution factor is the numerical expression that quantifies the reduction in concentration when a stock solution is mixed with a solvent. It represents the ratio of the final volume to the initial volume of stock solution used.

How do I calculate the dilution factor?

The dilution factor is calculated by dividing the final solution volume (V₂) by the volume of stock solution used (V₁). For example, if you use 10 mL of stock in a final volume of 100 mL, the dilution factor is 10.

What's the difference between dilution factor and dilution ratio?

Dilution factor is expressed as a single number (e.g., 10×), while dilution ratio is expressed as a fraction or ratio (e.g., 1:10). Both describe the same relationship but use different notation formats.

What does a 1:20 dilution factor mean?

A 1:20 dilution means 1 part of stock solution is mixed with 19 parts of diluent to create 20 parts total. This results in a 20-fold reduction in concentration from the original stock solution.

How do I dilute a solution by a factor of 10?

To achieve a 10× dilution, take 1 part of your stock solution and add 9 parts of diluent. For example, mix 10 mL of stock with 90 mL of diluent for a total volume of 100 mL.

Can I use different units for stock and final volumes?

Yes, this calculator allows you to select different units for stock and final volumes. It automatically converts between units (fL, pL, nL, μL, mL, L) to calculate the correct dilution factor.

What is the diluent volume?

Diluent volume is the amount of solvent (usually water or buffer) that needs to be added to the stock solution to achieve the desired final volume. It equals the final volume minus the stock volume.