Enter your Initial Concentration (C1), Final Concentration (C2), and Final Volume (V2) into the Drug Dilution Calculator to find the exact Stock Volume Needed (V1), plus the Solvent Volume to Add and Dilution Factor — so you can prep your solution without the back-of-napkin math.
Stock Volume Needed (V1)
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Solvent Volume to Add
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Dilution Factor
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This dilution formula calculates the volumes and concentrations needed when diluting solutions. C1 and V1 represent the initial concentration and volume, while C2 and V2 represent the final concentration and volume.
Enter your stock concentration (C1), desired final concentration (C2), and final volume (V2). The calculator will determine the volume of stock solution needed (V1) using the formula V1 = (C2 × V2) ÷ C1.
The calculator supports various concentration units including mg/mL, μg/mL, g/mL, and molar units (mM, μM, nM). Make sure both C1 and C2 use the same type of units for accurate calculations.
The solvent volume equals the final volume minus the stock volume (V2 - V1). This is the amount of diluent (usually water or buffer) you need to add to reach your desired final volume.
The dilution factor shows how much the original solution is diluted. It's calculated as the initial concentration divided by the final concentration (C1/C2). A 10:1 dilution factor means the solution is 10 times more dilute.
Yes, you can use this calculator for each step of a serial dilution. For each subsequent dilution, use the previous dilution's final concentration as the new C1 value.
If the calculated stock volume is too small to measure accurately (less than 1 μL), consider making a larger final volume or creating an intermediate dilution first to make the volumes more manageable.