Enter your DNA Amount (ng) and Number of Bases to convert between nanograms and picomoles — the Nanograms to Picomoles Converter handles both directions based on your chosen Conversion Direction, returning the Converted Amount alongside the calculated Molecular Weight and the exact Conversion Formula used.
Converted Amount
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Molecular Weight
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Conversion Formula
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The molecular weight of single-stranded DNA is calculated as 330 Da × number of bases. This uses the average molecular weight of a nucleotide (330 Daltons) multiplied by the sequence length.
The formula is: pmol ssDNA = [ng ssDNA / (330 × # of bases)] × 1000. This accounts for the molecular weight and unit conversion from grams to picomoles.
Single-stranded DNA uses 330 Da per base, while double-stranded DNA uses approximately 660 Da per base pair. This calculator is specifically designed for ssDNA and primers.
Picomoles are convenient units for DNA because they represent manageable numbers for typical laboratory quantities. They convert easily to other units and are commonly used in molecular biology applications.
Laboratory DNA concentrations typically range from nanograms to micrograms, with primer concentrations often in the picomole range. This calculator handles values from 0.0000001 ng to 10,000,000 ng.
Yes, this calculator is designed for both single-stranded DNA and primers. Both use the same molecular weight calculation based on the number of nucleotide bases in the sequence.
The 330 Da value is an average molecular weight for nucleotides and provides sufficient accuracy for most laboratory calculations. Individual nucleotides vary slightly, but this average is widely accepted in molecular biology.