Converting picomoles of DNA to nanograms requires knowing your sequence length — a step that trips up many lab workflows. Enter your DNA length (nucleotides), amount in picomoles, and DNA type (ssDNA or primer/oligonucleotide) into the Picomoles to Nanograms Converter to get the mass in nanograms. Secondary outputs include the calculated molecular weight and mass in micrograms.
Mass in Nanograms
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Molecular Weight
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Mass in Micrograms
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Both use the same calculation method since primers are single-stranded DNA oligonucleotides. The average molecular weight per nucleotide for ssDNA is approximately 330 g/mol.
The calculation uses an average molecular weight of 330 g/mol per nucleotide for ssDNA, which provides good accuracy for most applications. For precise work, consider the exact base composition.
Mass (ng) = Amount (pmol) × Molecular Weight (g/mol) × 10⁻³. The molecular weight equals the number of nucleotides × 330 g/mol for ssDNA.
This calculator is specifically designed for single-stranded DNA and primers. For dsDNA, use approximately 660 g/mol per base pair instead of 330 g/mol per nucleotide.
The calculator works for DNA lengths from 1 to 10,000 nucleotides, covering short primers to long oligonucleotides and single-stranded DNA fragments.
Nanograms are commonly used in molecular biology because typical working amounts of primers and short DNA fragments are in the nanogram range, making the units more convenient.