Enter your mRNA Sequence, choose a Genetic Code and Reading Frame, and toggle Start/Stop Codon Detection to let the mRNA to Protein Calculator decode your sequence into an Amino Acid Sequence with Protein Length, Molecular Weight, and codon counts.
Amino Acid Sequence
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Protein Length
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Estimated Molecular Weight
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Start Codons Found
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Stop Codons Found
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mRNA translation converts messenger RNA sequences directly into amino acid sequences using the genetic code. DNA must first be transcribed to mRNA before translation can occur.
Use the standard genetic code for most organisms. Choose mitochondrial codes for organellar genes, and organism-specific codes like mycoplasma for specialized bacteria.
Reading frames determine which nucleotides are grouped into codons. There are three possible frames, and choosing the wrong frame will produce completely different amino acid sequences.
Start codons (usually AUG) initiate protein synthesis, while stop codons (UAA, UAG, UGA) terminate translation. These define the boundaries of protein-coding sequences.
Premature stop codons can indicate frame shifts, mutations, or that you're not in the correct reading frame. Check your sequence and try different frames.
Only use A, U, G, and C nucleotides for mRNA sequences. The tool will ignore spaces and other characters, but ensure your sequence is accurate.
The molecular weight is estimated based on standard amino acid masses. For precise calculations, consider post-translational modifications and cofactors not included in this basic estimate.