Enter your Viable Cell Count, Dead Cell Count, Squares Counted, Dilution Factor, and Suspension Volume into the Viable Cell Count Calculator to find your Cell Viability (%), Viable Cell Concentration, Total Concentration, and Total Viable Cells.
Cell Viability
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Viable Cell Concentration
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Total Cell Concentration
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Total Viable Cells
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Trypan blue is a vital dye that cannot penetrate healthy cell membranes but enters dead or damaged cells, staining them blue. Viable cells remain unstained and appear bright under the microscope.
Counting multiple squares (typically 4 or 5) provides better statistical accuracy and reduces counting error. It gives a more representative sample of your cell suspension.
The standard dilution factor for trypan blue staining is 2:1 (equal volumes of cell suspension and 0.4% trypan blue), giving a dilution factor of 2.
Generally, >90% viability is excellent, 80-90% is good, 70-80% is fair, and <70% is poor. However, acceptable viability depends on your specific application and cell type.
The standard hemocytometer factor is 10,000 (1×10⁴), which converts the average cells per large square to cells per mL, accounting for the chamber's volume and dimensions.
Yes, this calculator works for any cell type that can be stained with trypan blue, including mammalian cells, yeast, and some bacterial cells.
The calculator accounts for the number of squares counted in its calculations. You can select 1, 4, or 5 squares depending on your counting method.