Enter your current column dimensions (length, diameter, particle size), flow rate, injection volume, and max pressure into the HPLC Method Calculator, then provide new column dimensions to get the scaled flow rate, adjusted injection volume, and estimated pressure for your method transfer.
New Flow Rate
--
New Injection Volume
--
Estimated Pressure
--
Runtime Change
--
HPLC method transfer is the process of adapting an existing chromatographic method to run on different instrumentation, column dimensions, or particle sizes while maintaining equivalent separation performance.
The new flow rate is calculated based on the ratio of column volumes and particle sizes between the old and new columns. This maintains similar linear velocity and separation efficiency.
Injection volume should be proportional to the column volume to maintain peak capacity and avoid overloading. Smaller columns typically require smaller injection volumes.
Pressure changes result from differences in column dimensions and particle sizes. Smaller particles and longer columns increase backpressure, while larger diameters decrease it.
Yes, methods can be transferred between HPLC and UHPLC systems by adjusting flow rates, injection volumes, and gradient conditions to account for different column specifications and system capabilities.
Pressure estimates are based on theoretical calculations and typical system parameters. Actual pressures may vary depending on mobile phase viscosity, temperature, and instrument-specific factors.