Enter your Molecular Mass, Charge State (z), and m/z Value into the Native Mass Spectrometry Calculator, choose your Calculation Type and toggle Include Adduct Correction with an optional Adduct Mass, and get your Primary Result alongside Secondary Calculation and Mass per Charge Unit for fast, fuss-free native MS analysis.
Primary Result
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Secondary Calculation
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Mass per Charge Unit
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Native mass spectrometry is a technique that analyzes biomolecules in their native, folded state under non-denaturing conditions. It preserves protein complexes and non-covalent interactions, allowing measurement of intact molecular masses.
The m/z ratio is calculated by dividing the molecular mass by the charge state: m/z = (Mass + z × Adduct Mass) / z, where z is the charge state and adduct mass accounts for ionization (typically 1.0078 Da for H+).
Native MS typically produces lower charge states compared to denaturing conditions. Proteins often show charge states between +5 to +30, depending on their size, with larger proteins generally having higher charge states.
Adduct mass correction accounts for the mass of ionizing species (like H+, Na+, or NH4+) that attach to the molecule during ionization. This correction is essential for accurate mass determination from m/z measurements.
Native MS can achieve mass accuracy within 0.01-0.1% for well-resolved peaks. The accuracy depends on instrument calibration, resolution, and the complexity of the charge state distribution.
Higher charge states result in lower m/z values for the same molecule. Multiple charge states of the same molecule appear as a series of peaks with predictable m/z spacing, allowing charge state determination.