Enter your Mass in Vial, choose your Mass Unit, and set your Desired Concentration to find exactly how much solvent to add — the Resuspension Calculator also returns your Final Stock Concentration and Total Final Volume, with an optional Molecular Weight field if you need molar units.
Volume of Solvent to Add
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Final Stock Concentration
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Total Final Volume
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Resuspension involves dissolving dried or lyophilized material (like oligos) in a solvent to create a stock solution. Dilution involves taking an existing solution and adding more solvent to reduce its concentration.
TE Buffer (10mM TrisHCl / 1mM EDTA) at pH 8.0 is recommended for most oligos. DNase-free water can also be used, but TE buffer provides better stability and prevents degradation.
The calculator handles unit conversions automatically. For manual conversion: 1 μM = 1000 nM = 1,000,000 pM. Mass-based units require molecular weight for conversion to molarity units.
When converting between mass-based units (mg, μg, ng) and molarity units (μM, nM, pM), molecular weight is required. The relationship is: Molarity = (mass in grams) / (molecular weight × volume in liters).
Most oligo suppliers provide molecular weight on the product sheet. For estimation, single-stranded DNA oligos are approximately 330 g/mol per nucleotide, and RNA oligos are about 340 g/mol per nucleotide.
Yes, the calculator works for any dried or lyophilized biomolecule where you know the mass and desired final concentration. Just ensure you use the appropriate molecular weight and solvent.
Store resuspended oligos at -20°C for long-term storage or 4°C for short-term use. Avoid repeated freeze-thaw cycles by making aliquots. Most oligos are stable for months when properly stored.
Common stock concentrations are 100 μM for most applications, though this varies by experiment. Higher concentrations (up to 1 mM) save storage space but may have solubility issues with some sequences.