Enter your DNA Length (in base pairs) and DNA Amount (in micrograms) to calculate the Picomole Ends in your sample — plus the Number of Molecules and Molar Concentration so you have the full picture of what you're working with.
Picomole Ends
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Number of Molecules
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Molar Concentration
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The formula is: pmol DNA = (µg DNA × 10^6 pg/µg) / (DNA length in bp × 660 pg/pmol). The value 660 pg/pmol represents the average molecular weight of a nucleotide pair in double-stranded DNA.
660 pg/pmol is the standard average molecular weight of a double-stranded DNA nucleotide pair, accounting for the molecular weights of all four bases (A, T, G, C) and their complementary pairs in a statistical average.
Picomole ends refers to the number of DNA molecule ends in picomoles. Since each double-stranded DNA molecule has two ends, this calculation determines the molar amount of DNA molecules present.
This calculator is most accurate for double-stranded DNA. For single-stranded DNA, RNA, or DNA with unusual base compositions, the 660 pg/pmol average may not be as precise, but it provides a good approximation for most applications.
This conversion is commonly used in molecular cloning, PCR setup, DNA ligation calculations, and determining molar ratios for enzymatic reactions where precise DNA concentrations are critical.
The number of molecules shows how many individual DNA molecules are present in your sample. This is calculated using Avogadro's number (6.022 × 10^23) to convert from moles to actual molecule count.
µg/ml represents mass concentration (weight per volume), while pmol/µl represents molar concentration (number of molecules per volume). Molar concentration is more useful for stoichiometric calculations in molecular biology.
Yes, this calculator works well for plasmid DNA. Simply enter the total length of the plasmid in base pairs and the amount in micrograms to get the picomole concentration of plasmid molecules.