Reconstitution — the process of dissolving a dry powdered compound into a liquid solvent — requires precise volume calculations to hit a target concentration. Enter the mass in vial (in mg, µg, or ng) and your desired concentration into the Reconstitution Calculator to find exactly how much solvent to add. Results include the volume to add in both mL and µL, plus the confirmed final concentration.
Volume to Add
--
Volume to Add (µL)
--
Final Concentration
--
To calculate reconstitution volume, divide the mass of substance in your vial by your desired final concentration. The formula is: Volume = Mass ÷ Desired Concentration. Make sure your units are compatible.
These represent different concentration scales: mg/mL (milligrams per milliliter) is 1000x more concentrated than µg/mL (micrograms per milliliter), which is 1000x more concentrated than ng/mL (nanograms per milliliter).
The amount of bacteriostatic water depends on your vial's peptide mass and desired concentration. Use this calculator to determine the exact volume needed. Common concentrations range from 0.5-2 mg/mL for most peptides.
Yes, this reconstitution calculator works for any lyophilized substance including peptides, proteins, antibodies, and other biologics. Just enter the mass in your vial and your target concentration.
If the calculated volume is very small (under 50 µL), consider using a lower target concentration to increase the volume, or use a precision pipette. You can also make a stock solution at higher concentration and dilute further.
Reconstituted peptides typically last 2-4 weeks when stored at 2-8°C with bacteriostatic water, or up to 6 months when frozen at -20°C in aliquots. Always check the manufacturer's recommendations for specific storage requirements.
For peptides and proteins, use sterile bacteriostatic water, which contains 0.9% benzyl alcohol as a preservative. For immediate use, sterile water for injection is also acceptable. Avoid tap water or non-sterile solutions.
Manufacturers may suggest reconstitution volumes based on typical use cases or stability considerations. Our calculator provides the exact mathematical volume for your desired concentration, which may differ from general recommendations.