Enter your Stock Concentration (C₁), Final Concentration (C₂), and Final Volume (V₂) into the Solution Dilution Calculator to find the Stock Volume Needed (V₁) — plus the Diluent Volume to Add and Dilution Factor so you know exactly how much to mix.
Stock Volume Needed
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Diluent Volume to Add
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Dilution Factor
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The dilution equation relates the concentration and volume of a stock solution to the concentration and volume of the final diluted solution. C₁ is the stock concentration, V₁ is the volume of stock needed, C₂ is the final concentration, and V₂ is the final volume.
First calculate the required stock volume using this calculator. Then pipette that volume of stock solution into a container, and add diluent (usually water or buffer) to reach the final desired volume. Mix thoroughly.
The calculator supports molarity units (M, mM, μM, nM, pM, fM), mass/volume units (mg/mL, μg/mL, ng/mL, pg/mL, g/L), and percentage concentration. Ensure both stock and final concentrations use the same unit type.
The dilution factor represents how many times the original solution is diluted. For example, a 10:1 dilution factor means the final solution is 10 times more dilute than the stock solution.
Yes, you can leave any one field blank and the calculator will determine that value based on the dilution equation. This works for stock concentration, stock volume, final concentration, or final volume.
For most laboratory applications, measure volumes to 3 significant figures. Use appropriate pipettes or graduated cylinders based on the volumes calculated. Very small volumes may require serial dilutions.
Final volume is the total volume of the diluted solution. Diluent volume is the amount of solvent you need to add to the stock solution to reach that final volume. Diluent volume = Final volume - Stock volume.